|
KMID : 0363320050260010060
|
|
Journal of Korean Oriental Internal Medicine
2005 Volume.26 No. 1 p.60 ~ p.73
|
|
|
The Effects of Loranthus parasiticus Merr. on Cell Cycle and Expression of Related Genes in HepG2 Cell
|
|
Rhew Kwang-Yul
Kim Young-Chul
Lee Jang-Hoon
Woo Hong-Jung
|
|
|
Abstract
|
|
|
Objectives : The aim of this study was to evaluate the effects of Loranthus parasiticus Merr. on cell cycle and expression of related genes in HepG2 cells.
Methods : The MTT assay, cell counting assay, [3H]-Thymidine incorporation assay, flow cytometric analysis, quantitative RT-PCR and western blot assay were studied.
Results : In the water extract of Loranthus parasiticus Merr., inhibition of cell proliferation and DNA synthesis in HepG2 cells was seen. These inhibitory effects were due to inhibition of G l-S transition in cell cycle. After treatment with the extract, expression of cyclin D1(G1 check point related gene) was inhibited particularly in dose-dependent and time-dependent manners.
Conclusion : These results suggest that the inhibition of cell cycle progression by Loranthus parasiticus Merr. in HepG2 cell is due to suppression of cyclin D1(G1 check point related gene) mRNA expression and protein synthesis.
|
|
|
KEYWORD
|
|
|
Loranthus parasiticus Merr, HepG2 cell, Cell cycle, Cyclin D1
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|